📋 Summary
This study by Hori, T. & Kurosawa, O. A Three-Dimensional Cell C was published in Tissue Engineering Part C: Methods 24, 730–739, 2018. It utilized Microtissues 3D Petri Dish® micro-molds for 3D cell culture, contributing to advances in developmental biology research.
ulture Method with a Micromesh Sheet and Its Application to Hepatic Cells
Hori, T. & Kurosawa, O. A Three-Dimensional Cell Culture Method with a Micromesh Sheet and Its Application to Hepatic Cells. Tissue Engineering Part C: Methods 24, 730–739 (2018)
Research Overview
This publication by Hori, T. & Kurosawa, O. A Three-Dimensional Cell C represents important research in the field of developmental biology. Published in Tissue Engineering Part C: Methods 24, 730–739, 2018, this work employed 3D Petri Dish® micro-mold technology from Microtissues to create uniform, reproducible 3D microtissues for their experimental studies.
🔬 3D Culture Approach
- Utilized Microtissues 3D Petri Dish® micro-molds for reproducible 3D spheroid formation
- Enabled physiologically relevant cell-cell interactions in a controlled 3D environment
- Supported the study of complex biological processes that cannot be replicated in traditional 2D culture
How 3D Petri Dish® Enabled This Research
🟢 3D Petri Dish® Application
The researchers chose Microtissues 3D Petri Dish® micro-molds to generate uniform 3D microtissues, enabling more physiologically relevant experimental conditions compared to traditional 2D cultures.
- Non-adhesive hydrogel micro-molds promoted self-assembly of cells into 3D spheroids
- Uniform microtissue size ensured experimental reproducibility
- Compatible with standard cell culture workflows and imaging techniques
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FAQs
3D Petri Dish® micro-molds are non-adhesive hydrogel molds that allow cells to self-assemble into uniform, reproducible 3D microtissues (spheroids). They are compatible with standard cell culture protocols and support a wide range of cell types and applications.
3D cell culture provides a more physiologically relevant environment compared to traditional 2D culture. Cells in 3D form natural cell-cell interactions, develop gradients of nutrients and oxygen, and better mimic in vivo tissue architecture — all critical for developmental biology studies.