Distributed Worldwide by Sigma-Aldrich

The Scaffold-Free 3D Cell Culture Platform

The 3D Petri Dish® enables researchers to create hundreds of uniform spheroids, organoids, and microtissues in a single pipetting step — no synthetic scaffolds, no gels, no animal products.

200+
Peer-Reviewed Papers
20+
Cell Types Validated
100+
Universities Worldwide
81–256
Spheroids Per Well

Why Researchers Choose the 3D Petri Dish®

Uniform Spheroids

Hundreds of identically sized spheroids formed in one pipetting step for reproducible, high-throughput experiments.

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Scaffold-Free

Cell-to-cell adhesion drives natural self-assembly. No synthetic scaffolds to alter cell physiology or confound results.

Reusable & Affordable

Autoclavable micro-molds fit standard 12- and 24-well dishes. Save thousands compared to consumable alternatives.

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Imaging-Ready

Spheroids settle on the same optical plane for easy, high-content microscopy without repositioning.

Featured in Leading Journals

Selected highlights from over 200 publications in Nature, Science, Cell, and more.

Immuno-Oncology

Pancreatic Cancer Immunotherapy with 3D Spheroids

Nature Communications · 2024

Patient-derived pancreatic tumor spheroids cultured in 3D Petri Dishes enabled testing of CAR-T cell immunotherapy, revealing how the tumor microenvironment affects immune cell infiltration.

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Cardiac Regeneration

Nanowired Cardiac Organoids for Heart Repair

Science Advances · 2023

Gold nanowire-enhanced cardiac organoids grown in 3D Petri Dishes showed improved electrical coupling and contractile function, advancing cardiac tissue engineering for heart repair.

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Developmental Biology

Retinal Organoid Development Atlas

Nature Biotechnology · 2023

Comprehensive single-cell atlas of retinal organoid development using 3D Petri Dish microwells, mapping cell type emergence and gene expression dynamics during human eye formation.

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Cancer Biophysics

Cancer Mechanomics & Machine Learning

Science Advances · 2024

Machine learning analysis of cancer spheroid mechanical properties in 3D Petri Dishes revealed distinct biomechanical signatures that correlate with tumor aggressiveness and drug resistance.

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Trusted by Leading Institutions

Columbia University
MIT
Brown University
Baylor College of Medicine

The 3D Cell Culture Technology

The 3D Petri Dish® is the natural 3D cell culture environment that is scaffold-free and maximizes cell-to-cell interactions. When cells are seeded onto the 3D Petri Dish, cell-to-cell adhesion drives self-assembly of the 3D microtissue, typically in 1–2 days.

  • Make hundreds of spheroids of uniform size in a single pipetting step
  • Control spheroid size precisely
  • Image an array of spheroids on the same optical plane
  • Form spheroids of two different cell types
  • Grow spheroids from single cell clones
  • Harvest spheroids for RT-PCR and Western Blots without enzymes
  • Form microtissues in complex shapes for novel applications
View Protocols
3D Petri Dish micro-molds on plate

Ready to Go 3D?

Join researchers at 100+ institutions who have made the switch to scaffold-free 3D cell culture.

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