📋 Summary
This study by Coyle, R., Yao, J., Richards, D. & Mei, Y was published in Tissue Eng Part A 25, 620–631, 2019. It utilized Microtissues 3D Petri Dish® micro-molds for 3D cell culture, contributing to advances in stem cell biology research.
The Effects of Metabolic Substrate Availability on Human Adipose-Derived Stem Ce
Coyle, R., Yao, J., Richards, D. & Mei, Y. The Effects of Metabolic Substrate Availability on Human Adipose-Derived Stem Cell Spheroid Survival. Tissue Eng Part A 25, 620–631 (2019). --- PAGE 20 ---
Research Overview
This publication by Coyle, R., Yao, J., Richards, D. & Mei, Y represents important research in the field of stem cell biology. Published in Tissue Eng Part A 25, 620–631, 2019, this work employed 3D Petri Dish® micro-mold technology from Microtissues to create uniform, reproducible 3D microtissues for their experimental studies.
🔬 3D Culture Approach
- Utilized Microtissues 3D Petri Dish® micro-molds for reproducible 3D spheroid formation
- Enabled physiologically relevant cell-cell interactions in a controlled 3D environment
- Supported the study of complex biological processes that cannot be replicated in traditional 2D culture
How 3D Petri Dish® Enabled This Research
🟢 3D Petri Dish® Application
The researchers chose Microtissues 3D Petri Dish® micro-molds to generate uniform 3D microtissues, enabling more physiologically relevant experimental conditions compared to traditional 2D cultures.
- Non-adhesive hydrogel micro-molds promoted self-assembly of cells into 3D spheroids
- Uniform microtissue size ensured experimental reproducibility
- Compatible with standard cell culture workflows and imaging techniques
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FAQs
3D Petri Dish® micro-molds are non-adhesive hydrogel molds that allow cells to self-assemble into uniform, reproducible 3D microtissues (spheroids). They are compatible with standard cell culture protocols and support a wide range of cell types and applications.
3D cell culture provides a more physiologically relevant environment compared to traditional 2D culture. Cells in 3D form natural cell-cell interactions, develop gradients of nutrients and oxygen, and better mimic in vivo tissue architecture — all critical for stem cell biology studies.