📋 Summary
This study by Dissanayaka, W. L., Zhu, L., Hargreaves, K. M., Jin, L. & Zhang, C was published in 2015. It utilized Microtissues 3D Petri Dish® micro-molds for 3D cell culture, contributing to advances in developmental biology research.
In Vitro Analysis of Scaffold-free Prevascularized Microtissue Spheroids Contain
Dissanayaka, W. L., Zhu, L., Hargreaves, K. M., Jin, L. & Zhang, C. In Vitro Analysis of Scaffold-free Prevascularized Microtissue Spheroids Containing Human Dental Pulp Cells and Endothelial Cells. Journal of Endodontics 41, 663–670 (2015)
Research Overview
This publication by Dissanayaka, W. L., Zhu, L., Hargreaves, K. M., Jin, L. & Zhang, C represents important research in the field of developmental biology. Published in 2015, this work employed 3D Petri Dish® micro-mold technology from Microtissues to create uniform, reproducible 3D microtissues for their experimental studies.
🔬 3D Culture Approach
- Utilized Microtissues 3D Petri Dish® micro-molds for reproducible 3D spheroid formation
- Enabled physiologically relevant cell-cell interactions in a controlled 3D environment
- Supported the study of complex biological processes that cannot be replicated in traditional 2D culture
How 3D Petri Dish® Enabled This Research
🟢 3D Petri Dish® Application
The researchers chose Microtissues 3D Petri Dish® micro-molds to generate uniform 3D microtissues, enabling more physiologically relevant experimental conditions compared to traditional 2D cultures.
- Non-adhesive hydrogel micro-molds promoted self-assembly of cells into 3D spheroids
- Uniform microtissue size ensured experimental reproducibility
- Compatible with standard cell culture workflows and imaging techniques
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FAQs
3D Petri Dish® micro-molds are non-adhesive hydrogel molds that allow cells to self-assemble into uniform, reproducible 3D microtissues (spheroids). They are compatible with standard cell culture protocols and support a wide range of cell types and applications.
3D cell culture provides a more physiologically relevant environment compared to traditional 2D culture. Cells in 3D form natural cell-cell interactions, develop gradients of nutrients and oxygen, and better mimic in vivo tissue architecture — all critical for developmental biology studies.