📋 Summary
This study by Kofron, C. M. et al was published in 2017. It utilized Microtissues 3D Petri Dish® micro-molds for 3D cell culture, contributing to advances in cardiac research.
Gq-Activated Fibroblasts Induce Cardiomyocyte Action Potential Prolongation and
Kofron, C. M. et al. Gq-Activated Fibroblasts Induce Cardiomyocyte Action Potential Prolongation and Automaticity in a Three-Dimensional Microtissue Environment. American Journal of Physiology-Heart and Circulatory Physiology 313, H810–H827 (2017). DOI: 0.1152/ajpheart.00181.2017
Research Overview
This publication by Kofron, C. M. et al represents important research in the field of cardiac. Published in 2017, this work employed 3D Petri Dish® micro-mold technology from Microtissues to create uniform, reproducible 3D microtissues for their experimental studies.
🔬 3D Culture Approach
- Utilized Microtissues 3D Petri Dish® micro-molds for reproducible 3D spheroid formation
- Enabled physiologically relevant cell-cell interactions in a controlled 3D environment
- Supported the study of complex biological processes that cannot be replicated in traditional 2D culture
How 3D Petri Dish® Enabled This Research
🟢 3D Petri Dish® Application
The researchers chose Microtissues 3D Petri Dish® micro-molds to generate uniform 3D microtissues, enabling more physiologically relevant experimental conditions compared to traditional 2D cultures.
- Non-adhesive hydrogel micro-molds promoted self-assembly of cells into 3D spheroids
- Uniform microtissue size ensured experimental reproducibility
- Compatible with standard cell culture workflows and imaging techniques
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FAQs
3D Petri Dish® micro-molds are non-adhesive hydrogel molds that allow cells to self-assemble into uniform, reproducible 3D microtissues (spheroids). They are compatible with standard cell culture protocols and support a wide range of cell types and applications.
3D cell culture provides a more physiologically relevant environment compared to traditional 2D culture. Cells in 3D form natural cell-cell interactions, develop gradients of nutrients and oxygen, and better mimic in vivo tissue architecture — all critical for cardiac studies.