3D Cell Culture Videos

Big Breakthroughs – The 3D Petri Dish Helps to Change Rhode Island

 

Casting, Equilibrating and Seeding the 3D Petri DishTM

Steps – Casting, Equilibrating and Seeding the 3D Petri DishTM

1. Pouring the gel
a. To the micro-mold, add agarose gel solution dissolved in saline.
2. Removal of the 3D Petri DishTM
a. After agarose solidifies, carefully remove the formed 3D Petri DishTM from the micro-mold, placing it in the well of a culture plate.
3. Equilibrating the 3D Petri DishTM
a. Add to the well the desired culture media, incubate for approximately 15 minutes to equilibrate.
b. If needed, remove any bubbles that block micro-wells.
4. Seeding 3D Petri DishTM with cells
a. Remove culture media around the 3D Petri DishTM, very carefully remove media inside seeding chamber.
b. Carefully add trypsinized cells to seeding chamber.
c. Let sit for 10 minutes to allow cells to settle into micro-wells, carefully add additional cell culture medium. Return to incubator.
5. Spheroids formed by the 3D Petri DishTM.

 

Title: Time Lapse Video of Self-Assembly of Small MSC Spheroids on the 3D Petri DishTM

Human mesenchymal stem cells (MSCs) were seeded on the 3D Petri DishTM used to form small spheroids (12-256).

Time lapse video of phase contrast images of self-assembly over 20 hours.

 

Time Lapse Video of Self-Assembly and Self-Sorting of Spheroids on the 3D Petri DishTM

A mix (1:1) of fibroblasts labeled red and hepatocytes labeled green were seeded on the 3D Petri DishTM

Time lapse fluorescent images (red & green) were obtained over 15 hours.

Fibroblasts (red) form inner core and hepatocytes (green) form outer coating of spheroids.

 

Time Lapse Video of Self-Assembly of Large MSC Spheroids on the 3D Petri DishTM

Human mesenchymal stem cells (MSCs) were seeded on the 3D Petri DishTM used to form large spheroids (12-81).

Time lapse video of phase contrast images of self-assembly over 20 hours.